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Do PathoSEEK® qPCR Assays work on the Thermofisher QuantStudio™ instruments?

PathoSEEK® qPCR assays WILL work on Quant Studio instruments, but certain settings and calibrations are required, and Medicinal Genomics will be limited with what support and troubleshooting it can provide.

All PathoSEEK® assays contain HEX and FAM dyes. Some assays can detect more than one pathogen. These multiplexed assays contain more dyes to distinguish different organisms. For example our Salmonella/E.coli multiplex assay contains HEX, FAM, and ROX dyes. As noted below in the systems dyes chart from the QuantStudio™ 3 and 5 installation manual, HEX requires custom dye calibration.

MGC's 5-color Aspergillus assay for the CFX96 will work with the QuantStudio™ 5. This assay contains the five dyes highlighted in the graph below. Screen Shot 2020-07-08 at 3.34.51 PM

If your lab has a ThermoFisher QuantStudio™ 5  they should order the Bio-rad version of our 5-color Aspergillus kit based on this info from the QuantStudio™ 5 manual.  The AriaMX version of the 5-color contains an ATTO 425 labeled probe, which is not listed in any of the QuantStudio™ documentation.

Thermo Calibrated dyes and PathoSEEK Aspergillus probe:
  • Aspergillus fumigatus: FAM probe
  • Aspergillus niger: ROX probe 
  • Aspergillus flavus: Cy5 probe
  • Aspergillus terreus: Cy 5.5 probe (will need customer calibration)
  • Cannabis Internal Control: HEX probe (will need custom calibration)