---
title: Can I test seeds?
description: At this time we do not have a validated protocol for prepping seeds for testing and we do not accept seeds as a sample type for genomic services.
---

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# Can I test seeds?

## At this time we do not have a validated protocol for prepping seeds for testing and we do not accept seeds as a sample type for genomic services.

We have not yet developed a reliable/scalable protocol for extracting gDNA from seed. The primary reason for this is that we want avoid testing, genotyping or sequencing the endosperm.  The seed coat of cannabis seeds, as in most flowering plants, is Maternal DNA. The Megagametophyte in the seed (endosperm) is pollinated with a different pollen than the Embryo. 

See here for more detail: https://genomebiology.biomedcentral.com/articles/10.1186/gb-2002-3-9-reviews1026

 

If you put seeds in ddH20 for a few days at 37 C, the root will emerge and enrich for embryo /endosperm ratio. We have PCR’d these for sex but this is destructive to the sample.

 

We do not have this ironed out as a protocol that could feed StrainSEEK®, Whole Genome, or CannSNP90 as those protocols require much more DNA.

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