---
title: Do PathoSEEK® qPCR Assays work on the Thermofisher QuantStudio™ instruments?
description: PathoSEEK® qPCR assays WILL work on Quant Studio instruments, but certain settings and calibrations are required, and Medicinal Genomics will be limited with what support and troubleshooting it can provide.
---
[Skip to content](https://help.medicinalgenomics.com/en/do-pathoseek-qpcr-assays-work-on-the-thermo-quant-studio-series#main-content)
[](https://medicinalgenomics.com)
- [Help Center](https://help.medicinalgenomics.com)
- [Web Store](https://store.medicinalgenomics.com)
Open main navigation
Close main navigation
- [Help Center](https://help.medicinalgenomics.com)
- [Web Store](https://store.medicinalgenomics.com)
- [Contact us](mailto:support@medicinalgenomics.com)
[Contact us](mailto:support@medicinalgenomics.com)
How can we help you?
- There are no suggestions because the search field is empty.
1. [Help Center](https://help.medicinalgenomics.com/en?hsLang=en)
2. [Microbial Safety Testing](https://help.medicinalgenomics.com/en/microbial-safety-testing?hsLang=en)
# Do PathoSEEK® qPCR Assays work on the Thermofisher QuantStudio™ instruments?
## PathoSEEK® qPCR assays WILL work on Quant Studio instruments, but certain settings and calibrations are required, and Medicinal Genomics will be limited with what support and troubleshooting it can provide.
All PathoSEEK® assays contain HEX and FAM dyes. Some assays can detect more than one pathogen. These multiplexed assays contain more dyes to distinguish different organisms. For example our Salmonella/E.coli multiplex assay contains HEX, FAM, and ROX dyes. As noted below in the systems dyes chart from the QuantStudio™ 3 and 5 installation manual, HEX requires custom dye calibration.
MGC's 5-color Aspergillus assay for the CFX96 will work with the QuantStudio™ 5. This assay contains the five dyes highlighted in the graph below. 
If your lab has a ThermoFisher QuantStudio™ 5 they should order the Bio-rad version of our 5-color Aspergillus kit based on this info from the QuantStudio™ 5 manual. The AriaMX version of the 5-color contains an ATTO 425 labeled probe, which is not listed in any of the QuantStudio™ documentation.
Thermo Calibrated dyes and PathoSEEK Aspergillus probe:
- *Aspergillus* fumigatus: FAM probe
- *Aspergillus* niger: ROX probe
- *Aspergillus* flavus: Cy5 probe
- *Aspergillus* terreus: Cy 5.5 probe (will need customer calibration)
- Cannabis Internal Control: HEX probe (will need custom calibration)
Link to Thermo user guide: [https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017162\_QS5HIDInstrument\_UG.pdf](https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017162_QS5HIDInstrument_UG.pdf)
- [Frequently Asked Questions](https://help.medicinalgenomics.com/en/frequently-asked-questions?hsLang=en)
- [Product Updates](https://help.medicinalgenomics.com/en/product-updates?hsLang=en)
- [Sample Purification](https://help.medicinalgenomics.com/en/sample-purification?hsLang=en#main-content)
- [Grim Reefer Free DNA Removal](https://help.medicinalgenomics.com/en/sample-purification?hsLang=en#grim-reefer-free-dna-removal)
- [Internal Control](https://help.medicinalgenomics.com/en/sample-purification?hsLang=en#internal-control)
- [AOAC](https://help.medicinalgenomics.com/en/sample-purification?hsLang=en#aoac)
- [Early Detection Plant Screening](https://help.medicinalgenomics.com/en/early-detection-plant-screening?hsLang=en#main-content)
- [FemINDICAtor® Plant Gender Testing](https://help.medicinalgenomics.com/en/early-detection-plant-screening?hsLang=en#femindicator-plant-gender-testing)
- [PathoSEEK® Plant Pathogen Testing](https://help.medicinalgenomics.com/en/early-detection-plant-screening?hsLang=en#pathoseek-plant-pathogen-testing)
- [Virus Assays](https://help.medicinalgenomics.com/en/early-detection-plant-screening?hsLang=en#virus-assays)
- [Genomic Services](https://help.medicinalgenomics.com/en/genomic-services?hsLang=en#main-content)
- [StrainSEEK®](https://help.medicinalgenomics.com/en/genomic-services?hsLang=en#strainseek)
- [Kannapedia](https://help.medicinalgenomics.com/en/genomic-services?hsLang=en#kannapedia)
- [Microbial Safety Testing](https://help.medicinalgenomics.com/en/microbial-safety-testing?hsLang=en#main-content)
- [Hardware and Consumables](https://help.medicinalgenomics.com/en/microbial-safety-testing?hsLang=en#hardware-and-consumables)
- [Validation](https://help.medicinalgenomics.com/en/microbial-safety-testing?hsLang=en#validation)
- [PathoSEEK](https://help.medicinalgenomics.com/en/microbial-safety-testing?hsLang=en#pathoseek)
- [Aspergillus](https://help.medicinalgenomics.com/en/microbial-safety-testing?hsLang=en#aspergillus)
- [Mic qPCR Instrument](https://help.medicinalgenomics.com/en/mic-qpcr-instrument?hsLang=en)
- [AriaMx](https://help.medicinalgenomics.com/en/ariamx?hsLang=en)
- [Automation](https://help.medicinalgenomics.com/en/automation?hsLang=en)
- [Product Specifications](https://help.medicinalgenomics.com/en/product-specifications?hsLang=en)
- [Contact Support](mailto:support@medicinalgenomics.com)
- [Sign Up for Our Newsletter](https://share.hsforms.com/1rFnAme5nTYuGeU0IdkMQGA20xr2)
- [Join Us at CannMed 26](https://cannmedevents.com)
[](http://www.medicinalgenomics.com)
Medicinal Genomics Help Center
Copyright © 2026, Medicinal Genomics