Is the hops latent viroid assay available in a singleplex RT-qPCR assay?

The hops latent viroid assay is multiplexed with the lettuce chlorosis and cannabis cryptic virus assays. All 3 assays can be independently evaluated for the price of one, along with an internal control, in one run.

The PathoSEEK® Cannabis Virus Multiplex Detection Assay A is a reverse-transcriptase quantitative PCR (RT-qPCR or QRT-PCR) assay that screens for the presence of hops latent viroid (HLVd), lettuce chlorosis virus (LCV), and cannabis cryptic virus (CCV).

Why did Medicinal Genomics choose these three viruses to multiplex?

These three viruses are the only three viruses with published and peer reviewed data supporting their  being found in cannabis. These papers are linked below. 

Lettuce Chlorosis Virus Disease:  A New Threat to Cannabis Production

Cannabis Cryptic Virus is mistaken for "hemp streak" disease

HLVd and LCV are two of the most common viruses that affect cannabis growers. CCV is less known to the community and often misunderstood and misdiagnosed. We anticipate increased demand for both LCV and CCV testing and wanted to multiplex these assays in with the HLVd assay to offer three assays for the price of one.

Please see our case study for information about how this assay can help improve yield.

Do I have to run all three assays?

All three targets are tested in a single PCR reaction. Like all PathoSEEK® assays, cannabis control is multiplexed in and utilizes the HEX optical channel. If only one of the assays is needed and there is no interest in another, the user can simply skip adding the positive control for that assay, saving reagents. However, as we stated above, we expect the market to demand LCV and CCV testing in the future as more information and research about what viruses affect cannabis is circulated.

Am I paying three times as much?

No. A singleplex virus assay would not be three times less expensive. Offering these three assays as a multiplex allows us certain efficiencies in manufacturing and we pass those onto the consumer to be competitive with other virus testing technologies on the market.

Do you have other virus assays?

Yes. We have several others in development including (in no particular order):

  1. Tobacco Mosaic Virus
  2. Tobacco Streak Virus
  3. Hops Mosaic Virus
  4. Beat Curly Top Virus
  5. Tobacco Ring Spot Virus
  6. Cucumber Mosaic Virus
  7. Arabis Mosaic Virus

If there are other virus assays you're interested in that we should add to our list or you have any immediate need for any of the above please do not hesitate to reach out to us at and we can schedule a call with our team to discuss.

If you do not have a qPCR instrument in your lab, information about what else you need to get started can be found here.

All Medicinal Genomics reagents are manufactured in a facility certified to ISO 9001 standards. All lots of reagents are functionally tested in house and Certificates of Analysis (COAs) are available upon request.