If shipping delays result in reagents arriving without dry ice, they remain stable and suitable for use. Stability testing was performed under extreme conditions (10 days at elevated temperatures) which confirmed that the reagents continue to perform within established specifications.
How were the stability testing experiments set up?
Stability testing of Medicinal Genomics Pathoseek® Detection Assays was performed using the following products:
- Aspergillus 5 color Assays – Bio-Rad and Agilent Aria Versions
- Aspergillus v3 Positive Control
After initial thaw of fresh reagents they were stored at the following temperatures for 10 days:
- -20 C
- 25 C (room temp)
- 37 C (98.6 F)
After ten days, each assay was tested with the following DNA targets:
- Aspergillus species specific DNA (5uL @ 500copies/µL) – A. niger, A. flavus, A. fumigatus, and A. terreus
- Internal Control DNA freshly diluted to a 1:1 M dilution with nuclease free water
- Assay positive control diluted 1:10 (as per MGC user guide)
Note: Bio Rad 5 color assay was run on the Bio-Rad CFX96
Agilent 5 color assay was run on the Agilent AriaMX
Results:
The Cq values and amplitude of amplification curves demonstrate consistency across all three temperature storage conditions, confirming that no performance drift occurs when the assay is applied to species-specific genomic DNA.-
- 5 µL of gDNA, @ 500 copies/ µL spiked into each reaction
