Medicinal Genomics has conducted comprehensive stability studies demonstrating that PathoSEEK® Detection Assays maintain performance even after exposure to elevated temperatures for extended periods.
Reagent Stability and Shipping Delays
If shipping delays cause reagents to arrive without dry ice, they remain stable and fully suitable for use. Medicinal Genomics has conducted comprehensive stability studies demonstrating that PathoSEEK® Detection Assays maintain performance even after exposure to elevated temperatures for extended periods.
Stability Testing Overview
Stability testing for Medicinal Genomics PathoSEEK® Aspergillus 5-Color Detection Assays (Bio-Rad and Agilent Aria versions) and the Aspergillus v3 Positive Control was performed to simulate extreme shipping and storage conditions.
After an initial thaw of fresh reagents, aliquots were stored for 10 days at the following temperatures:
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–20 °C (recommended storage)
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25 °C (room temperature)
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37 °C (98.6 °F; elevated temperature)
Test Design and Procedure
Following the 10-day storage period, each assay was evaluated using the following DNA targets:
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Aspergillus species-specific DNA (5 µL @ 500 copies/µL): A. niger, A. flavus, A. fumigatus, and A. terreus
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Internal Control DNA, freshly diluted 1:1 M dilution with nuclease-free water
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Assay Positive Control, diluted 1:10 in accordance with the MGC User Guide
Instrument platforms:
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Bio-Rad 5-Color Assay → Bio-Rad CFX96
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Agilent 5-Color Assay → Agilent AriaMX
Results and Interpretation
Across all three temperature conditions, Cq values and amplification curve amplitudes remained consistent.
These findings confirm that no performance drift occurs, even after prolonged exposure to room or elevated temperatures.
Conclusion:
PathoSEEK® Detection Assays remain stable and reliable despite potential temperature fluctuations during transit, ensuring dependable results even if reagents arrive without dry ice.
Supporting Data
5 µL of gDNA, @ 500 copies/ µL spiked into each reaction
