---
title: PathoSEEK® qPCR Assay Stability Testing
description: Medicinal Genomics has conducted comprehensive stability studies demonstrating that PathoSEEK® Detection Assays maintain performance even after exposure to elevated temperatures for extended periods.
---
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# PathoSEEK® qPCR Assay Stability Testing
## Medicinal Genomics has conducted comprehensive stability studies demonstrating that PathoSEEK® Detection Assays maintain performance even after exposure to elevated temperatures for extended periods.
### Reagent Stability and Shipping Delays
If shipping delays cause reagents to arrive without dry ice, they remain **stable and fully suitable for use**. Medicinal Genomics has conducted comprehensive stability studies demonstrating that PathoSEEK® Detection Assays maintain performance even after exposure to elevated temperatures for extended periods.
### Stability Testing Overview
Stability testing for **Medicinal Genomics PathoSEEK® Aspergillus 5-Color Detection Assays** (Bio-Rad and Agilent Aria versions) and the **Aspergillus v3 Positive Control** was performed to simulate extreme shipping and storage conditions.
After an initial thaw of fresh reagents, aliquots were stored for **10 days** at the following temperatures:
- **–20 °C** (recommended storage)
- **25 °C** (room temperature)
- **37 °C** (98.6 °F; elevated temperature)
### Test Design and Procedure
Following the 10-day storage period, each assay was evaluated using the following DNA targets:
- **Aspergillus species-specific DNA** (5 µL @ 500 copies/µL): *A. niger, A. flavus, A. fumigatus,* and *A. terreus*
- **Internal Control DNA**, freshly diluted 1:1 M dilution with nuclease-free water
- **Assay Positive Control**, diluted 1:10 in accordance with the MGC User Guide
**Instrument platforms:**
- Bio-Rad 5-Color Assay → **Bio-Rad CFX96**
- Agilent 5-Color Assay → **Agilent AriaMX**
### Results and Interpretation
Across all three temperature conditions, **Cq values and amplification curve amplitudes** remained consistent.
These findings confirm that **no performance drift** occurs, even after prolonged exposure to room or elevated temperatures.
**Conclusion:**
PathoSEEK® Detection Assays remain **stable and reliable** despite potential temperature fluctuations during transit, ensuring dependable results even if reagents arrive without dry ice.
**Supporting Data**
5 µL of gDNA, @ 500 copies/ µL spiked into each reaction
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