Addressing frequently asked questions about the transition of MGC's RT-qPCR Master Mix v2 and qPCR Master Kit v3 to the new "Amplification Mix" format.
What's happening?
Medicinal Genomics is re-branding the RT-qPCR Master Mix v2 as "PathoSEEK Amplification Mix". The SKU 420207 is no longer available.
What products are impacted?
Amplification Mix will replace RT-qPCR Master Mix v2 for the following methods:
- PathoSEEK® Virus Multiplex Detection Assay A Kit - P/N 420507
- Assay ― 1 tube (Store at -15 to -20 °C). Expires 2 Years from Date of Manufacture.
- PathoSEEK Amplification Mix - 3 Vials - store lyophilized at room temperature until ready to use. Before first use rehydrate in 675 uL of Nuclease Free Water and swirl or gently tip mix. Unused resuspended master mix can be stored at -20°C for up to 3 months or at 4°C for up to 3 weeks.
- PathoSEEK® Hop Latent Viroid Detection Assay v2 Kit- P/N 420512
- HLVd Assay ― 1 tube (Store at -15 to -20 °C). Expires 2 Years from Date of Manufacture.
- PathoSEEK Amplification Mix - 3 Vials - store lyophilized at room temperature until ready to use. Before first use rehydrate in 675 uL of Nuclease Free Water and swirl or gently tip mix. Unused resuspended master mix can be stored at -20°C for up to 3 months or at 4°C for up to 3 weeks.
Why is Medicinal Genomics making this change?
The lyophilized enzyme can work as both a standard qPCR assay enzyme as well as a RT-qPCR assay enzyme. There are simply different protocols required for each. The re-branding as "Amplification Mix" clarifies that the item is universal across both assay types. The fact that the enzyme is lyophilized makes it much more attractive for shipping and storage.
What other products is Amplification Mix validated for use with?
We are validating the lyophilized RT-qPCR enzyme (Amplification Mix) for use not only with the virus/viroid assays that require an RT enzyme, but also with our standard qPCR assays when paired with MaGiC Lysis. These assays are sold in a kit with the Amplification Mix.
We have validated the following assays for use with the Amplification Mix:
- 420517 - Salmonella and STEC Multiplex
- 420525 - Salmonella and E. coli Multiplex
- Aspergillus
- 420527 - Listeria
- 420526 - Staph and Pseudomonas Multiplex
- 420507 - Hop Latent Viroid
- 420512 - Virus Multiplex Detection Assay: Hop Latent Viroid / Lettuce Chlorosis / Cannabis Cryptic Multiplex
As we validate and kit more of our assays with the Amplification Mix, we will update this help article.
Can I purchase Amplification Mix by itself?
In an effort to simplify our menu and the ordering process, we have begun packaging the PathoSEEK Amplification Mix with the RT-qPCR or qPCR assay and where applicable the MaGiC Lysis reagents as a kit instead of individually.
If you have reason to order the Amplification Mix by itself, please call or email us at (866)574-3582 or support@medicinalgenomics.com