---
title: What is new with the Hop Latent Viroid Detection Assay version 2?
description: MGC has released an updated HLVd Detection assay (v2) as well as an updated RT-qPCR Master Kit
---
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# What is new with the Hop Latent Viroid Detection Assay version 2?
## MGC has released an updated HLVd Detection assay (v2) as well as an updated RT-qPCR Master Kit
What Changed:
1. The PathoSEEK HLVd Detection Assay v2 includes optimized primer/probe concentrations and can detect down to as few as 7 genomic copies.
2. MGCs new Amplification Mix (previously called PathoSEEK RT-qPCR Master Kit) comes lyophilized and is more sensitive than the previous version. When ready to use, just add water.
3. MGC has released a new [Plant Sampling Guide](https://help.medicinalgenomics.com/hubfs/Customer%20Facing%20SOPs/Virus%20Assays/HLVD%20v2/Sample%20Preparation%20Guide.pdf?hsLang=en) that describes two Nucleic Acid Purification options:
1. Quick Lysis (SKU: 420240): Very similar to the Leaf Punch Lysis (SKU: 420208) product - LPL can be used interchangeably with Quick Lysis
2. PurePrep (SKU: 420031): Purifies and concentrates DNA and RNA for increased sensitivity
4. The HLVd v2 assay can be run on the Agilent AriaMX, the Bio-Rad CFX96 or the Mic qPCR instrument in conjunction with they Myra Liquid Handler. [Click here for additional Information on the Mic/Myra](https://medicinalgenomics.com/mic-myra/)
1. Using the Mic/Myra allows the use of smaller reaction volumes, resulting in 40% reduction in cost per sample
Relevant Links:
[HLVd v2 User Guide](https://help.medicinalgenomics.com/hubfs/Customer%20Facing%20SOPs/Virus%20Assays/HLVD%20v2/PathoSEEK%C2%AE%20Hop%20Latent%20Viroid%20Detection%20Assay%20Quick%20Guide.pdf?hsLang=en)
[HLVd v2 Validation Guide](https://help.medicinalgenomics.com/hubfs/Validation%20Documents/PathoSEEK%20Hop%20Latent%20Viroid%20Validation%20Document.pdf?hsLang=en)
[HLVd v2 Starter Bundle web store page](https://store.medicinalgenomics.com/PathoSEEK-Hop-Latent-Viroid-Starter?utm_campaign=Support%20Communication&utm_medium=email&_hsmi=285333367&_hsenc=p2ANqtz-9GqkjrD4d2txRWaAIvBQFSIw3tqAoxxvTmXBG_TEgy3memS-J8IyaRoz5WCCeTXZlvah8_gSEWCZSY13ghcFrEopPKT7yq-Su0dXlT0s5KfY_WHNQ&utm_content=285333367&utm_source=hs_email)
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