What SOP should I be following for each Medicinal Genomics microbial detection assay?

This article will provide information and links each assay User Guide or SOP

As MGC works to transition all assays through AOAC a few changes are being made that will eventually apply to all assays:

  1. qPCR Master Kit v3 (Note:  Any pass fail assay can use v3 Master Mix)
  2. A 1 to 9 ratio of sample to TSB for all flower extractions  (Note:  Any pass file assay can use a 1 to 9 ratio)
  3. Updated conversion equations for all total tests
  4. Information on using commercially available CRMs/PTs with MGC Assays

Assays:

AOAC accredited Assays:

5 Color Aspergillus: USER GUIDE

  1. v3 MM flower and MIP/concentrate 
  2. 1:9 ratio for flower

Salmonella and STEC Multiplex AssayUSER GUIDE

  1. v3 MM flower and MIP/concentrate 
  2. 1:9 ratio for flower

Manufacturer Validated Assays:

2 Color Aspergillus:  

  1. v3 MM flower and MIP/concentrate 
  2. 1:9 ratio for flower

SenSATIVAx Extraction for Flower

SenSATIVAx Extraction for MIP

Agilent Aria

Pathoseek setup on Agilent Aria

Pathoseek Data Analysis on Agilent Aria

Bio-Rad CFX96

Pathseek setup on Bio-Rad CFX96

Pathseek Data Analysis on the Bio-Rad CFX96

Total Yeast and Mold: TYM User Guide

  1. v3 MM for flower and MIP/concentrate 
  2. 1:19 ratio for flower 

Total Aerobic Bacteria:  TAC User Guide

  1. v3 MM for flower and MIP/concentrate 
  2. 1:9 ratio for flower 

Total Coliform and Bile-Tolerant Gram Negative (BTGN)

  1. v2 MM for flower and MIP/concentrate 
  2. 1:14.2 ratio for flower 

SenSATIVAx Extraction for Flower

SenSATIVAx Extraction for MIP

Agilent AriaMX

Pathoseek setup on Agilent Aria

Pathoseek Data Analysis on Agilent Aria

Bio-Rad CFX96

Pathseek setup on Bio-Rad CFX96

Pathseek Data Analysis on the Bio-Rad CFX96

 

Link below to helpful conversion equation spreadsheet:

Conversion Equation Spreadsheet