What's new with the Pseudomonas and Staphylococcus Version 2 Assay?
January, 2025. Medicinal Genomics released a new Staphylococcus and Pseudomonas multiplex assay that detects each organism in one reaction on separate optical channels.
PathoSEEK® P. aeruginosa & S. aureus Multiplex Detection Assay v2 with MaGiC Lysis Kit (P/N 420526)
The PathoSEEK® P. aeruginosa & S. aureus Multiplex Detection Assay v2 with MaGiC Lysis Kit (PN 420526) will differentiate between Staphylococcus and Pseudomonas in one qPCR reaction. Staphylococcus aureus is detected on the FAM channel and Pseudomonas aeruginosa is detected on the Cy5 channel.
MaGiC Lysis reagents and Amplification Mix will be included with the assay in the kit.
*New 2026 User Guide update now includes Buffered Peptone Water as an alternate enrichment media to Tryptic Soy Broth.
Please visit the webstore page here
Version 1 Assays Discontinued
In the previous version of the Staph/Pseudo Multiplex assay (PN 420138), both organisms were detected using the FAM channel. This limitation required the use of "singleplex" assays for additional DNA analysis to determine whether S. aureus or P. aeruginosa was present when a FAM signal was detected.
The Staph/Pseudo Multiplex assay (PN 420138), along with the individual Staph and Pseudo singleplex assays (P/Ns 420136 and 420137) are discontinued.
Validation
The new assay kit (PN 420526) has been validated for use with MGC's MaGiC Lysis, which offers a faster and more streamlined process compared to the previous SenSATIVAx method.
The new method also introduces Buffered Peptone Water as an alternative enrichment media to Tryptic Soy Broth and the below validation studies show their equivalence.
Finally, the method was thoroughly tested by a third party for USP equivalence and that report is available below.
For the most up to date user guide and validation information, please refer to our Product Literature page here.
Compatibility
The new v2 assay and amplification mix are compatible with the SenSATIVAx DNA Purification kit. If the preferred DNA Isolation method is SenSATIVAx, the Staph and Pseudo Multiplex v1 Assay Kit (with Amplification Mix) can be ordered without MaGiC Lysis. SenSATIVAx will need to be ordered separately. Please contact support@medicinalgenomics.com if you have any questions.