On June 16th, 2022 MGC released an updated workflow for TYM testing. This included a new User Guide and a new Validation document. What changed?
- Flower samples and marijuana-infused products (MIPs) samples now both require the use of qPCR Master Kit v3 (SKU 420201)
- The upfront dilution has been changed for flower testing to 1g sample and 19 mLs TSB (instead of 1 g sample and 9 mLs previously)
- A heating step has been added to the DNA Purification process for MIP samples to aid in complete cell lysis
- This step requires a heat block capable of maintaining 65℃ (Recommended product from VWR)
- Both Flower and MIP Cq to CFU conversion equations have been updated and now require an additional back-calculation to account for the revised upfront dilution
- No new reagents are required