MGCs Grim Reefer Free DNA Removal Enzyme cannot be used in the Total Yeast and Mold Extraction Process. The MGC lysis buffer is used to inactivate the GR enzyme, however this will also inactivate the MGC-TLP enzyme (aids in digestion of yeast cells ) before it has a chance to do its job. The centrifugation step after removing 1 mL of sample from the WP bag into a 1.5 mL tube will preferentially pellet live cells over free floating DNA. We are working on an alternative to GR for this assay that will completely rid the sample of extracellular DNA and work with the TLP enzyme.