Why is the Grim Reefer free DNA removal enzyme not compatible with the new TYM v2 Extraction Process?

You cannot use Grim Reefer with this new process because the MGC lysis buffer addition is needed to inactivate the GR enzyme.  Unfortunately,  this will also inactivate the MGC-TLP enzyme before it has a chance to do its job.   However, the addition of the spin down step will preferentially pellet live cells over free floating DNA so this does aid in separating live cells from  dead DNA that will be left in the supernatant you are removing after the centrifugation step.   We hope to develop an alternative to GR for this assay that will completely rid the sample of dead DNA, but this may not happen for some time.