Certain PathoSEEK assays can be run on the iQ5, however none are compatible with the MyiQ.
BIO-RAD iQ5 can detect the fluorescence on FAM, HEX, Texas Red, and Cy5. That means any MGC single plex, or two-species multiplex assays (ex. Sal/STEC) as well as the grim reefer enzyme may be used on a BIO-RAD iQ5.
The MyiQ only detects fluorescence from FAM, therefore it is incompatible with all PathoSEEK assays.
More information about the BIO-RAD iQ cyclers can be found here.
- Aspergillus 5-color assay is not compatible with either of the BIO-RAD iQ systems.
- Labs using these thermocyclers will have to perform their own in-house experiments to create Cq to CFU conversion equations for microbial quantification. More information on Cq to CFU conversion equations can be found in appendix II in our Validation Document.