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Will the PathoSEEK® 5-Color Aspergillus Assay work on Thermofisher QuantStudio™ instruments?

Yes the 5 Color Aspergillus assay for the CFX96 will work in the QuantStudio™

If your lab has a ThermoFisher QuantStudio™ 5  they should order the Bio-rad version of our 5-color Aspergillus kit based on this info from the QuantStudio™ 5 manual.  The AriaMX version of the 5-color contains an ATTO 425 labeled probel, which is not listen in any of the QuantStudio™ documentation.

Below shows calibrated dyes are:
  • Aspergillus fumigatus:FAM probe(FAM Probe, detects fumigatus)
  • Aspergillus niger: ROX probe 
  • Aspergillus flavus:Cy5 probe
  • Aspergillus terreus: Cy 5.5 probe**
  • Cannabis Internal Control: HEX probe**
image-Jul-08-2020-05-20-52-35-AM
 
**HEX and Cy5.5 may need custom calibration.